CFP Applications
Protein and multiomics spatial applications with CFP
CFP mIF Assay
Multi-round 10-Plex FFPE Human normal tonsil section stained with CFP fluorophores. Panel A. Staining round 1 of 5 protein markers. After round 1 imaging was completed, all CFP signals were cleaved. Staining round 2 was then performed. Panel B. Staining round 2 of another 5 protein markers. CFP 440, 488, 555, 647 and 750 were used in this study.
RNA ISH Assay
While RNA ISH assays may be more expensive and complex than IHC/IF, its advanced capabilities and detailed insights into gene expression make it a valuable tool for many research and diagnostic applications.
- Leading RNA ISH assays are highly sensitive and specific for detecting target RNA molecules, even at low expression levels
- RNA ISH allows for studies on targets not suitable for using antibodies such as secreted molecules, non-coding RNA
- Detect viral and bacterial nucleic acids in tissue samples
- Biomarker validation on animal tissues samples antibody availability is limited
- Detection of pathogen and biomarkers on animal tissues.
FFPE HeLa cell pellet stained with Molecular Instruments 2-Plex HCR Pro with MALAT1 and PPIB probes
FFPE human normal breast stained with HCR Pro with PPIB probe
Protein/RNA Co-Detection Assay
Multi-round 20-plex CFP staining of 19 different protein markers and 1 RNA marker on the same FFPE human tonsil tissue section. Brightness was adjusted for visualization. Scale bars, 200 μm.
CFP enables multiomics detection combing multiplex protein detection and RNA detection from leading RNA ISH providers. The multiomics approach offers researchers flexibility in experiment designs and provides comprehensive multimodal information that helps unveil complex biological processes.
Spatial Transcriptomics and mIF Co-Detection Assay
CFP is compatible with 10x Genomics Xenium assays. It enables post-Xenium 10+ plex protein detection using the proprietary cleavable TSA based fluorophores.
*Post-Xenium slide was provided by Biochain
CFP multiomics applications:
Applied downstream of single-cell RNAseq, spatial transcriptomics and other spatial proteomics studies
Use RNA probes to replace unavailable or unspecific antibodies
Perform studies on targets not suitable for using antibodies such as secreted molecules
Offers an orthogonal way for biomarker co-expression and cross-validation in tumor micro-environment (TME), gene therapy and infectious diseases studies
